Effect of pH on the catalytic function and zinc content of native and immobilized anthrax lethal factor.
نویسندگان
چکیده
Translocation of the zinc-dependent metalloendopeptidase anthrax lethal factor (LF) from the endosome to the cytosol requires an acidic endosomal milieu. In the current study, we utilized immobilized (to prevent protein aggregation below pH 5.5) and native LF to assess the effect of pH on the function and metal content of LF. Our results reveal the diminution of LF's catalytic competence under moderately acidic conditions (pH ∼6) to be uncorrelated to the metal content of the protein. However, a significant degree of demetallation of LF (∼30%) was observed at pH values close to those found in late endosomes (pH ∼5), thus raising the possibility that a substantial proportion of LF molecules may not be in their zinc-bound state prior to translocation.
منابع مشابه
Influence of chemical denaturants on the activity, fold and zinc status of anthrax lethal factor
Anthrax lethal factor (LF) is a zinc-dependent endopeptidase which, through a process facilitated by protective antigen, translocates to the host cell cytosol in a partially unfolded state. In the current report, the influence of urea and guanidine hydrochloride (GdnHCl) on LF׳s catalytic function, fold and metal binding was assessed at neutral pH. Both urea and GdnHCl were found to inhibit LF ...
متن کاملConsequences and Utility of the Zinc-Dependent Metalloprotease Activity of Anthrax Lethal Toxin
Anthrax is caused by the gram-positive bacterium Bacillus anthracis. The pathogenesis of this disease is dependent on the presence of two binary toxins, edema toxin (EdTx) and lethal toxin (LeTx). LeTx, the major virulence factor contributing to anthrax, contains the effector moiety lethal factor (LF), a zinc-dependent metalloprotease specific for targeting mitogen-activated protein kinase kina...
متن کاملSynthesis of zinc oxide nanoparticle by zinc acetate precursor and study on its catalytic properties
ZnO nanoparticles have been prepared by a simple method in a short period of time. In this synthetic method, the sample was obtained using Zn(CH3COO)2·2H2O and a new template of hexamine salt. Their crystalline structure and morphology were studied by XRD and SEM. The optical properties of the sample were studied by UV–visible spectroscopy. The absorption spectr...
متن کاملحذف رنگ مستقیم سبز 6 با استفاده از نشانده شدن آنزیم لاکاس بر روی نانو ذرات فریت روی از محلول های آبی
Background and Objective: Manufactured wastewater management of industrial units containing toxic pollutants is essential for environmental protection. Considering the great applications and effects of using the nanomaterial and nanotechnology in the field of environmental protection, the nanoparticle of ZnFe2O4 has been used as a basic particle. On the other hand, enzyme processes, due to thei...
متن کاملSynthesis of zinc oxide nanoparticle by zinc acetate precursor and study on its catalytic properties
ZnO nanoparticles have been prepared by a simple method in a short period of time. In this synthetic method, the sample was obtained using Zn(CH3COO)2·2H2O and a new template of hexamine salt. Their crystalline structure and morphology were studied by XRD and SEM. The optical properties of the sample were studied by UV–visible spectroscopy. The absorption spectr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- FEBS letters
دوره 587 4 شماره
صفحات -
تاریخ انتشار 2013